There is an increasing demand for in vitro models that capture the function of living tissues in the field of medicine and life science. To capture the physiological function in engineered tissues, there is a need to create bio-structures that mimic the hierarchical architecture and complexity of living tissues. By exploiting innovative approaches including microfabrication techniques (MEMS) and material science, we focus on development of three-dimensional engineered tissues for regenerative medicine and fundamental life science.


Microvessel model

1. Microvessel model

Organ-on-a-chip is an innovative platform which incorporates cells of human origin. It can be used to fill the gap between non-clinical studies and clinical studies in drug development. This platform enables recreation of the dynamic and complex microenvironment and the structure of the tissue and hence will be useful for understanding human responses. Our lab aims to visualize disease mechanisms using microvessel model. This model allows us to take control of the spatial arrangement of the cells and other physical and biochemical factors. Microvessels respond in the manner of sprout formation or alteration in the barrier function depending on the added biochemical factors. We combine creation and visualization of the tissue to elucidate what is happening in our bodies.

ヒト培養細胞を用いた医薬品の有効性・安全性試験を可能とする新規評価系が求められており,この解決方法として近年注目されているのがorgan-on-a-chipとよばれるin vitro組織モデルです。その名のとおり,微小な臓器や組織がスライドグラスなどのチップ上に集積されたものを指し,マイクロ加工技術により,複雑で動的な生体の微小環境、構造および機能を再現する試みがなされています.コラーゲンゲルとマイクロ加工技術を用いた三次元微小血管構造形成により,血管新生と血管透過性の双方を評価しうる手法を開発しました。生体外モデルの利点は,その疾患現象に関わる細胞や物理的・化学的因子を任意に配置・変化させ,生体の中でのブラックボックスを細胞レベルで可視化できる点です.腫瘍組織内に血管組織が形成される様子,腫瘍細胞がその血管内へ浸潤する様子など,モデル組織の構築とイメージングを組み合わせ,生体内で起こっている現象を明らかにし,薬剤評価、効果的な治療法の確立,および疾患予防へと役立てることを目標としています.

Press release
E. Lee et al., J. Mater. Chem. B, 6, 1085 (2018)
J. Pauty et al, EBioMedicine, 27, 225-236 (2018)
H. Takahashi et al, Scientific Reports, 7, 42426 (2017)
J. Pauty & R. Usuba et al, Nanotheranostics, 1(1), 103-113 (2017)

Biomaterials as cell scaffold

2. Biomaterials as cell scaffold

Bundles of thin and multiply parallel fibers are widely seen in nature such as plant xylems, human protein assemblies and muscle/nerve tissues. Because of their hierarchical structure, they have superior toughness and strength relative to single fibers, and have been a key research focus in artificial tissue engineering. We have developed a new method to prepare biomimetic bundle-structured gel fibers using a microfluidic device and rapid cross-linking of a phase-separated polymer blend solution. The bundled gels have a feature of tunable surface topology and mechanical stiffness, and allow guiding cell orientation. These properties can be applicable for the cell culture scaffold, especially for muscle/nerve tissue regeneration.


Press release
S. Tachizawa, H. Takahashi et al, ACS Appl. Mater. Interfaces, 9(49), 43250-43257(2017)
Y.J. Kim et al, Biomaterials Science, 4, 1197-1201 (2016)
Y.J. Kim & Y. Takahashi et al, J. Mater. Chem. B, 3, 8154-8161 (2015).

Vascular Health

3. Vascular Health

It is important to keep blood vessel (capillaries) young in keeping the body healthy. Not only blood vessels are associated with transporting nutrients and oxygen but also they are involved in diseases. We investigate how healthiness of capillaries can be maintained by controlling food intake, exercise and environment.


4. Science x Design
科学 x デザイン

"Science for everyone!". In order to connect people and people through science, we conduct various expression activities through design. Our lab members have an opportunity to participate in the projects in RCA x IIS Tokyo Design lab.

科学を通じて人と人とがつながれるよう,デザインを通じたさまざまな表現活動を行っています.希望する研究室メンバーには,所内のRCA x IIS Tokyo Design labでのプロジェクトに参加していただいております.

RCA-IIS Tokyo Design Lab
血管の音色 Attune
RCA x IIS Tokyo Design lab

5. International collaboration

Our laboratory is a member of LIMMS, an international collaborative laboratory of CNRS and IIS. In 2014, SMMIL-E which is a new platform for the research on BioMEMS in Lille, France, has started.


SMMIL-E (English)
SMMIL-E (Japanese) "Spotlight on Technology Universities in Japan"